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When using a sucrose solution as a soluble nutrient source, the highest healing ratio (84.4%) was achieved after five days of incubation for concrete using the bio-self-healing bacteria Sporosarcina pasteurii. The bio-self-healing concrete formulation that did not include nutrient was not significantly different to the control concrete, indicating that the sucrose is necessary for the bacteria to metabolize the sucrose and cause the precipitation. However, the smaller effects of the nutrient in comparison to the cracked specimens in sand (80.7%) is likely attributed to the lower availability of pore-water and drying of the specimen during incubation.Figure 6: Healing ratio over time for ground environment specimens. The healing ratio was determined by the Image J software using 5 replicate measurements. The images were taken with a 20 objective lens. Each image was further analysed to determine the percentage of crack closure. The resulting healing ratio for each specimen was then used to determine the overall healing ratio of the specimens. Error bars represent standard error from the mean. Figure 7: Surface image of a ground environment specimen with (a) a cracked area after two days of incubation in water, and (b) after five days of incubation with a crack re-closed to 92% of the initial crack size.Figure 8: Slurry container showing the crack in the liquid substrate, with (a) microscope images showing the crack width and (b) a crack with a width of 1.34mm, after three days of incubation in water.Figure 9: Slurry container showing the cracked area after three days of incubation in a slurry with 30% clay content with (a) microscope images of the crack width and (b) cracked specimen with a crack width of 1.69mm.Figure 10: Slurry container showing the cracked area after three days of incubation with a slurry with (a) microscope images of the crack width, and (b) cracked specimen with a crack width of 1.35mm. Ground Environment Pro Fs2004 Crack Download The bio-self-healing concrete samples were prepared following standard procedures in [ 25 ], although in this study the shrinkage-free casting method was used. The main difference was the mixture of coarse aggregate, cement and the spores. The recipe for bio-self-healing concrete was given in Table 2. According to the procedures of [ 25 ], a solid suspension of spores was added to the mixture of cement and coarse aggregate during the mixing stage. Alginate was dissolved into a liquid paste. This paste was then mixed into the cement. The composition of bio-self-healing concrete was (kg/m3) coarse aggregate (200#), 100 m3 superplasticizer, 100 kg cement, and 20 kg liquid paste. The mixture was stirred for about 4 hours at room temperature before casting.
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